Long-acting glucagon analogue

In vitro potency and receptor binding data for NNC9204-0043 and native glucagon (unpublished data).

The reference compound to NNC9204-0043 is native glucagon (NNC0025-8000). Please indicate (with a check mark at ‘Please add the reference compound if available) during your compound request if you would like to have native glucagon (NNC0025-8000) included in your shipment.

Peptides and proteins have a tendency to adhere to glass and plastic surfaces. This may at low concentration impact the actual amount in solution. To minimize this unspecific adherence, adding detergents or inert proteins like e.g., ovalbumin or other serum albumins to the solution can minimize this phenomenon. In case albumins are added to peptide/protein solutions, ensure that the albumins are free of any proteases, but be aware that it will affect the apparent potency and affinity in in vitro assays in case a fatty acid is attached to the compound. The freeze-dried material should be stored at -18C. NNC9204-0043 can be dosed in vivo in a formulation vehicle containing 50mM sodium phosphate, 145mM propylene glycol, (0.007% polysorbate 20 if concentrations are so low that adsorption to vials may affect the concentration), pH 7.4. Stability in aqueous media is poor thus formulations should be used fresh. For in vitro it is recommended to dissolve human glucagon in 100% DMSO (e.g at a concentration of 300 uM) and keep at max 5C.